100 micron Zirconium Beads, Pre-filled Tubes

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$159.00 - $202.00

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Tougher than silica and less expensive than stainless steel, 100 µm Acid Washed Zirconium Beads (Zirconium Silicate), in pre-filled disruption tubes, are suitable for homogenizing bacteria, complex matrices like sludge, and small tissues. The 600 mg of zirconium beads have been optimized for efficient lysis of bacteria using high speed bead beaters. These tubes have been particularly useful of its application in protein misfolding cyclic amplification technique, which is used for replicating mammalian prions in vitro. The sensitivity of prion detection improved by 2-3 orders of magnitude in just one round.

The 100 micron Zirconium bead disruption tubes are available in Acid Washed and Molecular Biology Grade format. All beads are acid washed and baked. Molecular biology grade disruption tubes are made from beads baked twice and handled aseptically. Molecular Biology Grade Beads are certified nucleic acid, DNase, RNase, and protease-free. They are most appropriate for molecular biology applications, including PCR, which may be hindered by the presence of nucleases and/ or proteases.

Pre-Filled Tubes are a convenient solution for labs seeking time savings by no longer requiring researchers to weigh, fill and QC individual tubes. They are available in 2 mL skirted (i.e., self-standing) or unskirted polypropylene tubes to ensure compatibility with most tube homogenizers. Clear screw caps with O-rings included.

Citations

Brenner, M.; Lobel, L.; Borovok, I.; Sigal, N.; Herskovits, A. A. Controlled Branched-Chain Amino Acids Auxotrophy in Listeria monocytogenes Allows Isoleucine to Serve as a Host Signal and Virulence Effector. PLOS Genetics 2018, 14 (3), e1007283. https://doi.org/10.1371/journal.pgen.1007283. (RNA of Listeria monocylogenes is extracted to better understand pathogen virulence and host cell modulation crucial to novel drug development)

Geissler, M.; Beauregard, J. A.; Charlebois, I.; Isabel, S.; Normandin, F.; Voisin, B.; Boissinot, M.; Bergeron, M. G.; Veres, T. Extraction of Nucleic Acids from Bacterial Spores Using Bead-Based Mechanical Lysis on a Plastic Chip. Eng. Life Sci. 2011, 11 (2), 174–181. https://doi.org/10.1002/elsc.201000132. (Nucleic acid extraction and real-time PCR are enhanced using an integration bead-based mechanical cell lysis of Bacillus atrophaeus subsp. globigii onto a plastic chip)

Gonzalez-Montalban, N.; Makarava, N.; Ostapchenko, V. G.; Savtchenk, R.; Alexeeva, I.; Rohwer, R. G.; Baskakov, I. V. Highly Efficient Protein Misfolding Cyclic Amplification. PLOS Pathogens 2011, 7 (2), e1001277. https://doi.org/10.1371/journal.ppat.1001277. (Significant improvement in the process of replicating mammalian prions in vitro is achieved using Protein Misfolding Cyclic Amplification or PMCA)

Guo, B.; Manchester, M.; Luby, T.; Frigon, D. Composition of Heterotrophic Specialized Sub-Guilds Defined by a Positive RNA and Polyhydroxyalkanoate Correlation in Activated Sludge. Water Research 2018, 144, 561–571. https://doi.org/10.1016/j.watres.2018.07.059. (Nucleic acid extraction of bacteria from activated sludge system is required to highlight the significance of RNA and polyhydroxyalkanoate (PHA) as indicators of their metabolic activities)

Nguyen, T. G.; Vargas-Blanco, D. A.; Roberts, L. A.; Shell, S. S. The Impact of Leadered and Leaderless Gene Structures on Translation Efficiency, Transcript Stability, and Predicted Transcription Rates in Mycobacterium smegmatis. Journal of Bacteriology 2020. https://doi.org/10.1128/JB.00746-19 (Protein extraction of Mycobacterium smegmatis, a relative of the pathogen causing tuberculosis, is necessary to understand gene expression regulation)

Wüthrich, D.; Wenzel, C.; Bavan, T.; Bruggmann, R.; Berthoud, H.; Irmler, S. Transcriptional regulation of cysteine and methionine metabolism in Lactobacillus paracasei FAM18149. Front Microbiol 2018, 9. https://doi.org/10.3389/fmicb.2018.01261. (Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) of Lactobacillus paracasei is an essential tool to enhance the cheese flavor of bacteria by controlling the formation of volatile sulfur compounds)

Additional Info

pH_Fractionation_and_identification_of_proteins.pdf (622.5 KB)

Pre-filled_Tubes_Sales_Sheet.pdf (1.6 MB)

Prefilled_Tubes(US_ENG)_SDS.pdf (172.6 KB)

Probiotic_Metabolites_from_Bacillus_coagulans_GanedenBC30_support_maturation_of_antigen-presenting_cells_in_vitro.pdf (883.3 KB)

Zirconium_Grinding_Beads(US_ENG)_SDS.pdf (121.0 KB)

100 micron Zirconium Beads in a disruption tube

Tougher than silica, less expensive than stainless steel, and inert in most solutions, 100 µm Acid Washed Zirconium Beads (Zirconium Silicate) are suitable for homogenizing bacteria and small tissues.

Beads are available in Acid Washed, Low Binding, and and Molecular Biology Grade format. Since untreated beads are notorious for containing large amounts of debris and dust, all beads are acid washed, at a minimum, prior to packaging.

Low Binding Beads are chemically altered using a proprietary process to bind less biomolecules liberated from homogenized samples.

Molecular Biology Grade Beads are certified nucleic acid, Dnase, Rnase, and protease-free. They are most appropriate for molecular biology applications, including PCR, which may be hindered by the presence of nucleases and/ or proteases.

Citations

Brenner, M.; Lobel, L.; Borovok, I.; Sigal, N.; Herskovits, A. A. Controlled Branched-Chain Amino Acids Auxotrophy in Listeria Monocytogenes Allows Isoleucine to Serve as a Host Signal and Virulence Effector. PLOS Genetics 2018, 14 (3), e1007283. https://doi.org/10.1371/journal.pgen.1007283.

Wüthrich, D.; Wenzel, C.; Bavan, T.; Bruggmann, R.; Berthoud, H.; Irmler, S. Transcriptional Regulation of Cysteine and Methionine Metabolism in Lactobacillus Paracasei FAM18149. Front Microbiol 2018, 9. https://doi.org/10.3389/fmicb.2018.01261.