100 micron Zirconium Beads in a 1200 mg disruption tube
Tougher than silica, less expensive than stainless steel, and inert in most solutions, 100 µm Acid Washed Zirconium Beads are suitable for homogenizing bacteria and small tissues. May also be used as a sonication additive.
Beads are available in Acid Washed, Low Binding, and and Molecular Biology Grade format. Since untreated beads are notorious for containing large amounts of debris and dust, all beads are acid washed, at a minimum, prior to packaging.
Low Binding Beads are chemically altered using a proprietary process to bind less biomolecules liberated from homogenized samples.
Molecular Biology Grade Beads are certified nucleic acid, DNase, RNase, and protease-free. They are most appropriate for molecular biology applications, including PCR, which may be hindered by the presence of nucleases and/or proteases.
Pre-Filled Tubes are a convenient solution for labs seeking time savings by no longer requiring researchers to weigh, fill and QC individual tubes. They are available in 2 mL skirted (i.e., self-standing) or unskirted (i.e., conical) polypropylene tubes to ensure compatibility with most tube homogenizers. Clear screw caps with O-rings included.
Citations
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Nguyen, T. G.; Vargas-Blanco, D. A.; Roberts, L. A.; Shell, S. S. The Impact of Leadered and Leaderless Gene Structures on Translation Efficiency, Transcript Stability, and Predicted Transcription Rates in Mycobacterium Smegmatis. Journal of Bacteriology 2020. https://doi.org/10.1128/JB.00746-19
Brenner, M.; Lobel, L.; Borovok, I.; Sigal, N.; Herskovits, A. A. Controlled Branched-Chain Amino Acids Auxotrophy in Listeria Monocytogenes Allows Isoleucine to Serve as a Host Signal and Virulence Effector. PLOS Genetics 2018, 14 (3), e1007283. https://doi.org/10.1371/journal.pgen.1007283.
Wüthrich, D.; Wenzel, C.; Bavan, T.; Bruggmann, R.; Berthoud, H.; Irmler, S. Transcriptional Regulation of Cysteine and Methionine Metabolism in Lactobacillus Paracasei FAM18149. Front Microbiol 2018, 9. https://doi.org/10.3389/fmicb.2018.01261.
Guo, B.; Manchester, M.; Luby, T.; Frigon, D. Composition of Heterotrophic Specialized Sub-Guilds Defined by a Positive RNA and Polyhydroxyalkanoate Correlation in Activated Sludge. Water Research 2018, 144, 561–571. https://doi.org/10.1016/j.watres.2018.07.059.
Gonzalez-Montalban, N.; Makarava, N.; Ostapchenko, V. G.; Savtchenk, R.; Alexeeva, I.; Rohwer, R. G.; Baskakov, I. V. Highly Efficient Protein Misfolding Cyclic Amplification. PLOS Pathogens 2011, 7 (2), e1001277. https://doi.org/10.1371/journal.ppat.1001277.
Geissler, M.; Beauregard, J. A.; Charlebois, I.; Isabel, S.; Normandin, F.; Voisin, B.; Boissinot, M.; Bergeron, M. G.; Veres, T. Extraction of Nucleic Acids from Bacterial Spores Using Bead-Based Mechanical Lysis on a Plastic Chip. Eng. Life Sci. 2011, 11 (2), 174–181. https://doi.org/10.1002/elsc.201000132.
