OPS Diagnostics’ 5 mm 440 C Stainless Steel Grinding Balls can be used with 4 ml and 15 ml vials. These treated and ready-to-use 5 mm Grinding Balls are made of slightly magnetic steel, allowing them to be retrieved with a magnet, while remaining resistant to corrosion.
To develop and validate a high-throughput method for extracting DNA, these 5 mm grinding balls were used to homogenize root nodules of soybean and mung bean. The grinding balls were also used to homogenize the fungal pathogen Botrytis cinerea of blueberry for DNA extraction to profile the resistance of populations to various fungicides.
In entomology, the same balls were used to grind ticks to extract their DNA to investigate the infection rates of Rickettsia species in the Lone Star tick populations. To develop a rapid and cost-effective method for generating multi-locus barcoding data from large collections of arthropods, DNA extraction was performed using the 5 mm stainless steel grinding balls.
All OPS Diagnostics’ grinding balls are treated to remove residual oils and contaminants prior to packaging so they are ready-to-use.
Our 5 mm 440C Stainless Steel Grinding Balls are suitable for use with the Qiagen TissueLyser bead dispenser.
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Citations
de Kerdrel, G. A.; Andersen, J. C.; Kennedy, S. R.; Gillespie, R.; Krehenwinkel, H. Rapid and Cost-Effective Generation of Single Specimen Multilocus Barcoding Data from Whole Arthropod Communities by Multiple Levels of Multiplexing. Scientific Reports 2020, 10 (1), 1–12. https://doi.org/10.1038/s41598-019-54927-z. (Combining multiplex PCRs and DNA extractions from pools of pre-sorted and morphotyped specimens, along with two levels of sample indexing. reduce costs and effort by up to 50-fold)
Egizi, A.; Gable, S.; Jordan, R. A. Rickettsia Spp. Infecting Lone Star Ticks (Amblyomma Americanum) (Acari: Ixodidae) in Monmouth County, New Jersey. J Med Entomol 2020. https://doi.org/10.1093/jme/tjz251. (About 25.0% of the 1,858 nymphal Amblyomma americanum tested, were infected with Rickettsia amblyommatis)
Johnson, G. High Throughput DNA Extraction of Legume Root Nodules for Rhizobial Metagenomics. AMB Expr 2019, 9 (1), 47. https://doi.org/10.1186/s13568-019-0771-z. (The method was utilized on root nodules of soybean and mung bean that had been dehydrated with silica, yielding DNA that was concentrated, minimally fragmented, and devoid of contaminants such as phenols and polysaccharides)
Saito, S.; Michailides, T. J.; Xiao, C. L. Fungicide Resistance Profiling in Botrytis Cinerea Populations from Blueberry in California and Washington and Their Impact on Control of Gray Mold. Plant Disease 2016, 100 (10), 2087–2093. https://doi.org/10.1094/PDIS-02-16-0229-RE. (The intron Bcbi-143/144 intron in gene cytb was detected in 11.8% and 40% of the isolates in California and Washington, respectively, suggesting that the risk of QoI resistance is higher in California than in Washington)
