96 Well SYNERGY™ Plant DNA Extraction Kit

96 Well Synergy™ Plant DNA Extraction Kit
Back to List
$581.00
SKU: SYNP 02-96-03
Qty:

SYNERGY Banner

OPS Diagnostics’ SYNERGY™ DNA extraction platform includes a high throughput 96 well plate format for isolating high quality plant DNA in a timely and cost-effective manner without the use of hazardous chloroform.  The high throughput format is valuable to greatly accelerate scientific research and discovery.  This high throughput format and related automation allow for reliable testing, screening, and analysis of increasing large batches of samples while saving time and resources in processing.

Building upon the same proprietary CTAB nucleic acid isolation chemistry found in the original SYNERGY™ Plant DNA Extraction Kit, the 96 Well SYNERGY™ Plant DNA Extraction Kit includes materials to process two 96 Deep Well Plates (192 preps) of samples. This method utilizes dry filtering and binding plates to yield and purify genomic DNA of high quality and quantity for downstream applications. This high-throughput DNA extraction kit has a wide range of applications across many scientific disciplines and is an essential tool in molecular biology, genetics, and biochemistry laboratories. Some key applications are in agriculture, biotechnology, and genomics research.  In agriculture and biotechnology, this kit is used as a tool in plant pathogen detection, disease diagnoses, and breeding desirable crop traits such as increased yield, insect, pest and disease resistance. High-throughput kits are used to prepare DNA samples for next-generation sequencing, fundamental in genomic research. Unlike competing kits where sample preparation is sold separate to their DNA extraction protocols, the 96 Well SYNERGY™ Plant DNA Extraction Kit includes pre-filled deep well plates necessary for a bead beating homogenization step, allowing for high throughput sample processing with minimal variation in upstream sample preparation. Alternatively, eight tube strips can be removed from the plate to accommodate partial plate homogenization.  As many as 192 samples of any plant species or part (leaves, stem, roots, flowers, and fruits) or microorganisms can be processed in as little as two hours from a single kit.

The 96 Well SYNERGY™ Plant DNA Extraction Kit includes: two pre-filled 96 Well Homogenization Plates (each containing grinding resin and stainless steel grinding balls), two Well Support Mats, Plant Homogenization Buffer (100 ml), RNase A Solution (1800 μl), two Filter Plates, two Binding Plates, four Collection Plates w/lids, two Elution Plates w/lids, and instructions.

Related Literature

User Guide(pdf)

Protocol: High Throughput Isolation of Plant DNA using the 96 Well Synergy™ Plant DNA Extraction Kit

96 Well Synergy™ Plant DNA Extraction Kit Video

Product Information Sheet

Citations

Leopold, D. R.; Busby, P. E. Joint Effects of Host Genotype and Species Arrival Order Govern Microbiome Composition and Function. bioRxiv 2020, 2020.02.28.970582. https://doi.org/10.1101/2020.02.28.970582.

Noyszewski, A. K.; Anderson, N. O.; Smith, A. G.; Kilian, A.; Dalbotten, D.; Ito, E.; Timm, A.; Pellerin, H. Workshop: Challenges of Establishing Native versus Exotic Status of Herbarium Specimens. HortTechnology 2019, 1 (aop), 1–5. https://doi.org/10.21273/HORTTECH04313-19.

 


  • Description
  • Q&A

SYNERGY Banner

OPS Diagnostics’ SYNERGY™ DNA extraction platform includes a high throughput 96 well plate format for isolating high quality plant DNA in a timely and cost-effective manner without the use of hazardous chloroform.  The high throughput format is valuable to greatly accelerate scientific research and discovery.  This high throughput format and related automation allow for reliable testing, screening, and analysis of increasing large batches of samples while saving time and resources in processing.

Building upon the same proprietary CTAB nucleic acid isolation chemistry found in the original SYNERGY™ Plant DNA Extraction Kit,

The 96 Well SYNERGY™ Plant DNA Extraction Kit

includes materials to process two 96 Deep Well Plates (192 preps) of samples. This method utilizes dry filtering and binding plates to yield and purify genomic DNA of high quality and quantity for downstream applications. This high-throughput DNA extraction kit has a wide range of applications across many scientific disciplines and is an essential tool in molecular biology, genetics, and biochemistry laboratories. Some key applications are in agriculture, biotechnology, and genomics research.  In agriculture and biotechnology, this kit is used as a tool in plant pathogen detection, disease diagnoses, and breeding desirable crop traits such as increased yield, insect, pest and disease resistance. High-throughput kits are used to prepare DNA samples for next-generation sequencing, fundamental in genomic research. Unlike competing kits where sample preparation is sold separate to their DNA extraction protocols, the 96 Well SYNERGY™ Plant DNA Extraction Kit includes pre-filled deep well plates necessary for a bead beating homogenization step, allowing for high throughput sample processing with minimal variation in upstream sample preparation. Alternatively, eight tube strips can be removed from the plate to accommodate partial plate homogenization.  As many as 192 samples of any plant species or part (leaves, stem, roots, flowers, and fruits) or microorganisms can be processed in as little as two hours from a single kit.

 

The 96 Well SYNERGY™ Plant DNA Extraction Kit includes: two pre-filled 96 Well Homogenization Plates (each containing grinding resin and stainless steel grinding balls), two Well Support Mats, Plant Homogenization Buffer (100 ml), RNase A Solution (1800 μl), two Filter Plates, two Binding Plates, four Collection Plates w/lids, two Elution Plates w/lids, and instructions.

Related Literature

User Guide(pdf)

Protocol: High Throughput Isolation of Plant DNA using the 96 Well Synergy™ Plant DNA Extraction Kit

96 Well Synergy™ Plant DNA Extraction Kit Video

Product Information Sheet

Citations

Leopold, D. R.; Busby, P. E. Joint Effects of Host Genotype and Species Arrival Order Govern Microbiome Composition and Function. bioRxiv 2020, 2020.02.28.970582. https://doi.org/10.1101/2020.02.28.970582.

Noyszewski, A. K.; Anderson, N. O.; Smith, A. G.; Kilian, A.; Dalbotten, D.; Ito, E.; Timm, A.; Pellerin, H. Workshop: Challenges of Establishing Native versus Exotic Status of Herbarium Specimens. HortTechnology 2019, 1 (aop), 1–5. https://doi.org/10.21273/HORTTECH04313-19.

 


  • If I do not have 96 samples to prep, do I need to homogenize the full plate?

Answer:  No, the full plate does not need to be homogenized. Unused strips can be removed before homogenizing the plate. Take care to balance the plate, ensuring that both sides have relatively the same mass.

  • Can I use a vortexer instead of a plate homogenizer to homogenize the samples?

Answer:  No, a vortexer will not work.  We recommend a high velocity plate homogenizer to disrupt samples.

  • What do I do if my bottle of Plant Homogenization Buffer has white precipitates?

Answer:  The Plant Homogenization Buffer contains CTAB, which can precipitates out of solution when exposed to low temperatures. If necessary, warm the buffer in a water bath before using to ensure that the CTAB is in solution. 

  • How do I know that my samples have been adequately homogenized?

Answer:  The photo on the left shows a strip of tubes that have not been completely homogenized and should be homogenized longer, as indicated by the presence of foam. The photo on the right shows a strip of tubes that have been sufficiently homogenized.

Poor HomogenizationAdequate Homogenization

 

  • Can I use more than 40 mg of starting sample mass?

Answer:  Yes.  We recommend 40 mg of starting sample mass for optimal extractions, but starting mass can range from 30 mg to 50 mg.  We recommend increasing Plant Homogenization Buffer to 400 µl if starting mass is 50 mg.  

  • Can I use freeze-dried tissue?

Answer:  Yes.  We recommend using the equivalent of 40 mg of wet-weight tissue (i.e., approximately 10 mg of freeze-dried sample).  An additional 50 µl of Plant Homogenization Buffer can be added. 

  • What is the maximum volume of supernatant I can recover if I have more than 120 µl of supernatant after centrifuging the Homogenization Plate?

Answer:  We do not recommend exceeding 180 µl of supernatant to allow for the addition of isopropanol.

  • Should I use TE or Molecular Biology Grade Water as my elution buffer?

Answer:  If the elutions are being read on a spectrophotometer, we recommend eluting in MB water because EDTA in TE Buffer binds to the glass filter plates, altering  260/230 ratios.  We recommend adding final concentration 1X TE buffer, pH 8, to elutions for storage purposes.

  • Can I elute in more than 50 µl of buffer?

Answer: Our recommended range is between 50 µl - 100 µl of buffer.  Increased volume will result in lower DNA concentrations.

 

 

Products

Information