Freeze Drying Indicator is a colorimetric indicator useful in optimizing freeze drying runs by indicating sample dryness during both the primary and secondary drying steps. It is a pink solution that turns blue upon lyophilization, providing a real-time, visual measurement of dryness. When using this reagent, complete removal of water yields a blue cake, while incomplete removal of water is illustrated as pink streaks or a pink crust. Lyophilized control solutions that are pink contain >2% moisture, while solutions that are blue with residual pink color are <2% moisture.
OPS Diagnostics’ Freeze Drying Indicator is filter sterilized and packaged aseptically in 500 ml PET bottles. It can be used full strength or diluted 1:1 with samples prior to freeze drying.
For more information on lyophilization, please visit the following pages:
Lyophilization Reagents Shelf Life Study
Bacteria Lyophilization Overview
Bacteria Freeze Drying Protocol
Bushman, F.; Wu, G.; Lewis, J.; Goulian, M. Compositions and Methods Comprising a Defined Microbiome and Methods of Use Thereof. US20160243175A1, August 25, 2016.