D-Trehalose, Lyophilization Certified, is a lyoprotectant, which effectively maintains the conformation of proteins and other biomolecules as water is removed during the freeze-drying process. This non-reducing disaccharide (two glucose molecules linked by the anomeric carbon), provides the same type of protection during lyophilization that sucrose does, though D-Trehalose is less likely to cause sample collapse. D-Trehalose is a crystalline dihydrate powder with a molecular weight of 378.33 g/mol. It is soluble in water to 6.8” at 20° C. It is an effective lyoprotectant of proteins at a weight/volume concentration of 1%-2%.
Each lot of our D-Trehalose is tested to ensure that it is effective at preserving protein structure and activity. D-Trehalose is available with a Certificate of Analysis, attesting to the absence of proteases and nucleases and its ability to form a solid cake/matrix that can easily be rehydrated.
Please visit the following pages for additional information regarding other products used during lyophilization:
Lyophilization Reagent (2X), Filter Sterilized
Evaluation of Lyophilization Reagent Concentrate for the Preservation of LDH from Mouse Tissue Lysates