The HT Mini™’s compact design allows for rapid benchtop homogenization at a fraction of the cost of larger machines. The HT Mini™ accepts up to three 2 ml unskirted (i.e., conical bottom) screw capped microfuge tubes, offering a homogenization speed range from 2800 RPM to 4000 RPM. The digital readout allows precise speed configuration and timing. The HT Mini™ can homogenize bacteria, fungi and yeasts, human muscle biopsies, soft animal tissues (e.g., small and large intestines, skeletal muscle, dorsal root ganglion), and plant samples (e.g., duckweed, Arabidopsis seedlings, embryo and pericarp of field-grown rice plants).

Unsure of which pre-filled tubes to use with your samples? See our prefilled tube comparison paper. 

Not sure where to start? Check out our application table for a list of samples and a suggested homogenization method.

Citations

Gönczi, M., Ráduly, Z., Szabó, L., Fodor, J., Telek, A., Dobrosi, N., Balogh, N., Szentesi, P., Kis, G., Antal, M., Trencsenyi, G., Dienes, B., & Csernoch, L. (2022). Septin7 is indispensable for proper skeletal muscle architecture and function. eLife, 11. https://doi.org/10.7554/elife.75863

(Septin7 isoform is crucial for skeletal muscle function, with its knockdown causing deformities, reduced muscle force, and impaired cell division, indicating its role in muscle regeneration and development)

Huang, W., Gilbert, S., Poulev, A., Acosta, K., Lebeis, S., Long, C., & Lam, E. (2020). Host-specific and tissue-dependent orchestration of microbiome community structure in traditional rice paddy ecosystems. Plant and Soil, 452(1–2), 379–395. https://doi.org/10.1007/s11104-020-04568-3(The type of host tissue and the specific plant species are crucial factors in how microbes colonize plants in paddy fields)

Wang, R., Luo, S., Clarke, B. B., & Belanger, F. C. (2021). The Epichloë festucae antifungal protein Efe-AfpA is also a possible effector protein required for the interaction of the fungus with its host grass Festuca rubra subsp. rubra. Microorganisms, 9(1), 140. https://doi.org/10.3390/microorganisms9010140 (The antifungal protein gene Efe-afpA in Epichloë festucae is essential for disease resistance and symbiosis with strong creeping red fescue, as its absence causes disease susceptibility and failed infection)

Woodbury, T. J., Grush, E., Allan, M. C., & Mauer, L. J. (2021). The effects of sugars and sugar alcohols on the pasting and granular swelling of wheat starch. Food Hydrocolloids, 126, 107433. https://doi.org/10.1016/j.foodhyd.2021.107433 (Sorbitol, isomaltulose, and isomalt showed similar effects to sucrose, making them potential candidates for reduced-sugar baked goods)

Woodbury, T. J., Pitts, S. L., Pilch, A. M., Smith, P., & Mauer, L. J. (2022). Mechanisms of the different effects of sucrose, glucose, fructose, and a glucose–fructose mixture on wheat starch gelatinization, pasting, and retrogradation. Journal of Food Science, 88(1), 293–314. https://doi.org/10.1111/1750-3841.16414 (A 50:50 glucose:fructose mixture and sucrose solutions differently affect the gelatinization, pasting, and retrogradation properties of wheat starch, impacting the texture and shelf-life of baked goods)

Zhang, X., & Li, Y. (2023). Organ defects of the Usp7K444R mutant mouse strain indicate the essential role of K63-polyubiquitinated Usp7 in organ formation. Journal of Biological Chemistry, 298(4), 10104958.https://www.sciencedirect.com/science/article/pii/S2319417022000282 (K63-linked polyubiquitination of Usp7 is crucial for the development of various organs, highlighting the significance of this process in regulating essential protein functions)