OPS Diagnostics’ Synergy™ DNA extraction platform features proprietary technology to isolate high quality DNA in a timely and cost-efficient manner without the use of hazardous chloroform.
Building upon the same proprietary nucleic isolation chemistry found in the original Synergy™ Plant DNA Extraction Kit, the Synergy™ 2.0 Plant DNA Extraction Kit includes additional Silica Spin Columns to extract plant DNA for NGS and other downstream applications requiring the highest purity levels. Sample processing time is 45 minutes or less, with yields and purities exceeding those generated by traditional homegrown CTAB protocols and commercially-available kits.
An extensive analysis of the parameters for Synergy™ 2.0 can be found here. This study compares several DNA extraction methods and variables and demonstrates the advantages to adding spin columns to the standard Synergy™ protocol. Essentially, DNA yields and purity are improved over results achieved using simple alcohol precipitation (found in standard protocol).
DNA successfully extracted from plants including: Anthurium, Corn, Cotton, Grape, Pine Needles, Rape Seed, Rice, Rye, Sorghum, Soybean, Sunflower, and Wheat
The Synergy™ 2.0 Plant DNA Extraction Kit includes: 100 pre-filled Homogenization Tubes (each containing grinding resin and satellite), 100 Silica Spin Columns, Plant Homogenization Buffer (60 ml), RNase A Solution (600 µl), and instructions.
US Customers: Click HERE to request a free SYNERGY 2.0™ sample kit.
Product Information Sheet
Synergy™ 2.0 Application Note
Synergy™ 2.0 Research Note: Co-Isolation of Plant and Pathogen/Parasite DNA and Its Potential Use in Disease Detection and Monitoring
Zhao, M.; Dutta, B.; Luo, X.; Burdman, S.; Walcott, R. R. Genetically Distinct Acidovorax Citrulli Strains Display Cucurbit Fruit Preference under Field Conditions. PhytopathologyTM 2020. https://doi.org/10.1094/PHYTO-10-19-0389-R.
Wagner, M. R.; Roberts, J. H.; Balint-Kurti, P.; Holland, J. B. Microbiome Composition Differs in Hybrid and Inbred Maize. bioRxiv 2020, 2020.01.13.904979. https://doi.org/10.1101/2020.01.13.904979.
Mafune, K. K.; Godfrey, B. J.; Vogt, D. J.; Vogt, K. A. A Rapid Approach to Profiling Diverse Fungal Communities Using the MinIONTM Nanopore Sequencer. BioTechniques 2019, 68 (2), 72–78. https://doi.org/10.2144/btn-2019-0072.
Poulev, A.; Heckman, J. R.; Raskin, I.; Belanger, F. C. Tricin Levels and Expression of Flavonoid Biosynthetic Genes in Developing Grains of Purple and Brown Pericarp Rice. PeerJ 2019, 7, e6477. https://doi.org/10.7717/peerj.6477.
Wagner, M. R.; Busby, P. E.; Balint-Kurti, P. Breeding for Broad-Spectrum Disease Resistance Alters the Maize Leaf Microbiome. bioRxiv 2019, 647446. https://doi.org/10.1101/647446.