Responding to the growing demand for transportation of samples from fields to labs for molecular testing, OPS Diagnostics has devised the following protocol.
The protocol requires a small homogenizer to bead beat the samples in a buffer, preserving the integrity of the DNA until samples can be processed in a lab.
Protocol
Place samples in a 2 ml Pre-filled disruption tube with grinding media.
Add 500 µl of Plant Homogenization buffer, close the cap tightly.
Homogenize for 2 minutes on the highest speed.
The tubes can now be transported at ambient temperature upto 5 days.
When ready to process, transfer the contents of the tube to a Synergy™ Homogenization tube.
Homogenize for 1 minute on the highest speed.
Follow the Synergy™ protocol prescribed.
Results
Corn DNA purified, using the Rapid field Prep. Protocol. The purified DNA was analyzed using an Agilent Bioanalyzer 2100 (Agilent).
