The 50 ml polycarbonate vials with silicone-lined caps are useful for the homogenization of large and resilient samples, including animal tissues, leaves, and seeds. For example, the vials have been used to grind apple bark for extracting DNA of Erwinia amylovora, a plant pathogenic bacteria, and grasses (Bahiagrass, St. Augustinegrass leaves) to analyze their nutrient content. The 50 ml polycarbonate vials with silicone-lined caps have also been used to grind soybean root tissues to extract DNA of Fusarium brasiliense, a fungal pathogen and the causal agent of sudden death syndrome of soybean. During the homogenization process, the tissue samples of these plants were ground in a 50 ml polycarbonate vial containing grinding balls; a crucial step in releasing DNA for qPCR analysis. (Grinding balls sold separately.)
OPS Diagnostics' 50 ml polycarbonate vials are clear and extremely durable. They can be submerged in liquid nitrogen and immediately used for homogenizing. Their reinforced bottoms are specially designed to withstand the force generated by grinding balls during processing at cryogenic temperatures. Each vial comes with a silicone-lined cap, which can also be used for both cryogenic and room-temperature sample processing.
The 50 ml polycarbonate vials are available in cases of 100 vials, including silicone-lined caps. The vials fit into the standard 15 ml Cryoblocks (up to six vials per block) or OPS Diagnostics' foam vial holders (up to five vials per holder).
Vials can be used with the GenoGrinder® or MiniG™ high throughput homogenizers.
Related Literature
Selection of Grinding Vials for Sample Processing
Citations
Dhar, B. C., Santander, R. D., & Aćimović, S. G. (2024). Improved Canker Processing and Viability Droplet Digital PCR Allow Detection of Erwinia amylovora Viable Nonculturable Cells in Apple Bark. Microorganisms, 12(2), 376. https://doi.org/10.3390/microorganisms12020376 (This new method enables the detection of viable but nonculturable (VBNC) Erwinia amylovora cells in apple bark)
Maia, L. O. R., & Shaddox, T. W. (2019). Grinding methods influence nutrient analysis of bahiagrass and St. Augustine grass. Crop Science, 59(2), 787–791. https://doi.org/10.2135/cropsci2018.07.0466 (Different grinding methods significantly affect the nutrient analysis of bahiagrass and St. Augustinegrass)
Roth, M. G., Oudman, K. A., Griffin, A., Jacobs, J. L., Sang, H., & Chilvers, M. I. (2019). Diagnostic qPCR Assay to Detect Fusarium brasiliense, a Causal Agent of Soybean Sudden Death Syndrome and Root Rot of Dry Bean. Plant Disease, 104(1), 246–254. https://doi.org/10.1094/pdis-01-19-0016-re (This assay can differentiate Fusarium brasiliense from other similar species in clade 2 of the Fusarium solani species complex (FSSC) with a sensitivity threshold of 100 femtograms)
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