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Rat Feces Homogenization Protocol

Analysis of rat feces is commonly performed for pharmacokinetic analysis of new drugs.  Following collection, the initial step for analysis is to homogenize the sample.  Fortunately rat feces are relatively soft and grind easily.  However when multiple test subjects are evaluated, the grinding of the feces to a fine powder can be laborious, and potentially hazardous from dust formation.  High throughput homogenization can speed up sample processing while also containing the samples, thus reducing the risk of exposure.

    Protocol

  1. Homogenization can be performed using either the HT Homogenizer or GenoGrinder 2010.  Each homogenizer can hold 15 ml Vial Sets.  The HT Homogenizer having a capacity of 5 vials and the GenoGrinder 2010 having 10 vials, which can be expanded to 20 vials.

  2. One 15 ml vial can hold several grams of rat feces (30-40 droppings).  Remove the cap from the vial and add the feces.  The vial requires two grinding balls (7/16" stainless steel) which are supplied with the vial sets.  When using replacement vials, either re-use existing grinding balls or obtain new balls.  Replace the cap and set the vial sets into their foam rack.

  3. Remove the lid of the vial set and place the vial set into the homogenizer.  For the HT Homogenizer, adjust the latches on the lid to it securely holds the vials.  For the GenoGrinder 2010, place spacers on top of the vials so that they are secure when the lid in clamped into place.

  4. Process the rat feces at 1200 rpm for 2 min.  Remove the vials from the homogenizer.  Grinding balls can be removed using Magnet Tips

  5. Ground samples can be stored in the grinding vial until needed.

  6. Rat Feces Before Grinding

    Rat Feces in 15 ml Vial

    Rat Feces After Grinding