Rat Feces Homogenization Protocol
Analysis of rat feces is commonly performed for
pharmacokinetic analysis of new drugs. Following collection, the initial step for analysis is to homogenize the sample. Fortunately
rat feces are relatively soft and grind easily. However when
multiple test subjects are evaluated, the grinding of the feces to a fine
powder can be laborious, and potentially hazardous from dust formation.
High throughput homogenization can speed up sample processing while also
containing the samples, thus reducing the risk of exposure.
Protocol
- Homogenization can be performed using the HT Homogenizer
or GenoGrinder 2010. Each homogenizer can hold
15 ml Vial Sets. The HT Homogenizer having a capacity of 5 vials and
the GenoGrinder 2010 having 10 vials, which can be expanded to 20 vials.
- One 15 ml vial can hold several grams of rat feces (30-40 droppings). Remove the cap from the vial and add the feces.
The vial requires two grinding balls (7/16" stainless steel) which are supplied with the vial sets. When using replacement vials, either
re-use existing grinding balls or obtain new balls. Replace the cap and set the vial sets into their foam rack.
- Remove the lid of the vial set and place the vial set into the homogenizer. For the HT Homogenizer, adjust the latches on the
lid to it securely holds the vials. For the GenoGrinder 2010, place spacers on top of the vials so that they are secure when the lid in
clamped into place.
- Process the rat feces at 1200 rpm for 2 min. Remove the vials from the homogenizer. Grinding balls can be removed using
Magnet Tips.
- Ground samples can be stored in the grinding vial until needed.
Grinding Process
 Rat Feces Before Grinding |
 Rat Feces in 15 ml Vial |
 Rat Feces After Grinding |
